Precision In Vitro Solutions for Accelerated Discovery
At HT Biotechnology, our In Vitro Platform empowers researchers with cutting-edge cell-based technologies to streamline drug discovery, biomarker validation, and therapeutic development. From primary cell isolation to advanced gene editing, our GLP-compliant workflows deliver reliable, reproducible data to advance your projects from bench to bedside.
Core Services
Cell Culture & Model Development
- Primary Cell Isolation: Animal-derived cells (hepatocytes, neurons, immune cells) with >95% viability.
- Cell Line Development: Stable/transient transfection, CRISPR-edited cell lines, and 3D organoid models.
- Custom Co-Cultures: Tumor microenvironment, blood-brain barrier, and stromal-epithelial interactions.
Functional Cell Analysis
- Proliferation & Viability: MTT, CCK-8, colony formation assays.
- Cell Cycle & Apoptosis: Flow cytometry (PI/Annexin V), live-cell imaging.
- Migration & Invasion: Transwell, wound healing, spheroid invasion assays.
Genetic Engineering
- CRISPR/Cas9 Editing: Knockout, knock-in, and base editing with INDEL validation.
- RNAi & Overexpression: siRNA/shRNA libraries, lentiviral/AAV delivery.
- Reporter Cell Lines: Luciferase, GFP/RFP-tagged constructs for pathway analysis.
Flow Cytometry & Immunology
- Multiparametric Analysis: 15-color panels for immune profiling (T/B/NK cell subsets).
- Cell Sorting: FACS isolation of rare populations (stem cells, CTCs).
- Functional Assays: Cytokine release, phagocytosis, and ADCC/CDC evaluation.
